Adachi K, Tateishi M, Horii Y, Nagatomo H, Shimizu T, Makimura S
Department of Veterinary Internal Medicine, Faculty of Agriculture, Miyazaki University, Japan.
J Vet Med Sci. 1994 Oct;56(5):997-9. doi: 10.1292/jvms.56.997.
Reactivity of anti-erythrocyte membrane (RBCm) antibody in sera of B. gibosni-infected dogs on native RBCm and erythrocyte surface (RBCs) was examined using an enzyme linked immunosorbent assay. Anti-RBCm antibody attached to native RBCm and the surface of erythrocytes (RBC) treated with phenylhydrazine or neuraminidase, but not to the surface of non-treated RBC. Based on these results, anti-cytoskeletal protein antibody in sera of B. gibsoni-infected dogs is considered to attach to native RBCm, and furthermore free radical-induced oxidative stress imposed on RBC and sialic acid removal from glycoproteins of RBC are considered necessary for anti-transmembrane protein antibody in sera of B. gibsoni-infected dogs to be bound to RBCs. These are important to elucidating the mechanism of the marked increase in RBCs-bound IgG value and anemia in B. gibsoni infection.
使用酶联免疫吸附测定法检测了感染吉氏巴贝斯虫的犬血清中抗红细胞膜（RBCm）抗体对天然RBCm和红细胞表面（RBCs）的反应性。抗RBCm抗体附着于天然RBCm以及经苯肼或神经氨酸酶处理的红细胞（RBC）表面，但不附着于未处理的RBC表面。基于这些结果，认为感染吉氏巴贝斯虫的犬血清中的抗细胞骨架蛋白抗体附着于天然RBCm，此外，感染吉氏巴贝斯虫的犬血清中的抗跨膜蛋白抗体与RBC结合，红细胞上的自由基诱导的氧化应激以及红细胞糖蛋白上唾液酸的去除被认为是必要的。这些对于阐明吉氏巴贝斯虫感染时红细胞结合IgG值显著增加和贫血的机制很重要。
