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Approaches to teaching fluorescence spectroscopy.荧光光谱学的教学方法。
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Application of fluorescence spectroscopy for determining the structure and function of proteins.荧光光谱法在测定蛋白质结构与功能中的应用。
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The slow folding reaction of barstar: the core tryptophan region attains tight packing before substantial secondary and tertiary structure formation and final compaction of the polypeptide chain.巴氏杆菌抑制因子的缓慢折叠反应：在多肽链形成大量二级和三级结构并最终压实之前，核心色氨酸区域就已实现紧密堆积。
J Mol Biol. 2000 Sep 15;302(2):479-95. doi: 10.1006/jmbi.2000.4060.
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A study of density of states and ground states in hydrophobic-hydrophilic protein folding models by equi-energy sampling.通过等能抽样对疏水-亲水蛋白质折叠模型中的态密度和基态进行的一项研究。
J Chem Phys. 2006 Jun 28;124(24):244903. doi: 10.1063/1.2208607.
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Simulation of fluorescence resonance energy transfer experiments: effect of the dyes on protein folding.荧光共振能量转移实验模拟：染料对蛋白质折叠的影响。
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Studies of protein folding and dynamics using single molecule fluorescence spectroscopy.利用单分子荧光光谱法对蛋白质折叠和动力学的研究。
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Frustration and hydrophobicity interplay in protein folding and protein evolution.在蛋白质折叠和蛋白质进化过程中，挫折感与疏水性相互作用。
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Femtosecond studies of tryptophan fluorescence dynamics in proteins: local solvation and electronic quenching.蛋白质中色氨酸荧光动力学的飞秒研究：局部溶剂化与电子猝灭
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Exploring the binding pocket for pyridopyrimidine ligands at the CCK1 receptor by molecular docking.通过分子对接探索CCK1受体上吡啶并嘧啶配体的结合口袋。
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本文引用的文献

1
Time-resolved fluorescence of the single tryptophan of Bacillus stearothermophilus phosphofructokinase.嗜热脂肪芽孢杆菌磷酸果糖激酶单个色氨酸的时间分辨荧光
Biophys J. 1993 Jul;65(1):215-26. doi: 10.1016/S0006-3495(93)81070-1.
2
Fluorescence anisotropy assays implicate protein-protein interactions in regulating trp repressor DNA binding.荧光各向异性分析表明，蛋白质-蛋白质相互作用参与调节色氨酸阻遏蛋白与DNA的结合。
Biochemistry. 1993 Aug 3;32(30):7753-8. doi: 10.1021/bi00081a021.
3
Pressure stability of proteins.蛋白质的压力稳定性
Annu Rev Phys Chem. 1993;44:89-113. doi: 10.1146/annurev.pc.44.100193.000513.
4
The use of fluorescence methods to monitor unfolding transitions in proteins.使用荧光方法监测蛋白质的去折叠转变。
Biophys J. 1994 Feb;66(2 Pt 1):482-501. doi: 10.1016/s0006-3495(94)80799-4.
5
A three-dimensional model for the hammerhead ribozyme based on fluorescence measurements.基于荧光测量的锤头状核酶三维模型。
Science. 1994 Nov 4;266(5186):785-9. doi: 10.1126/science.7973630.
6
Time-resolved fluorescence of the two tryptophans in horse liver alcohol dehydrogenase.马肝醇脱氢酶中两个色氨酸的时间分辨荧光
Biochemistry. 1981 Jul 21;20(15):4369-77. doi: 10.1021/bi00518a021.
7
Quenching of protein fluorescence by oxygen. Detection of structural fluctuations in proteins on the nanosecond time scale.氧对蛋白质荧光的猝灭。在纳秒时间尺度上检测蛋白质中的结构波动。
Biochemistry. 1973 Oct 9;12(21):4171-9. doi: 10.1021/bi00745a021.
8
Time-resolved fluorescence of proteins.蛋白质的时间分辨荧光
Annu Rev Biochem. 1985;54:43-71. doi: 10.1146/annurev.bi.54.070185.000355.
9
Sugar transport by the bacterial phosphotransferase system. The intrinsic fluorescence of enzyme I.细菌磷酸转移酶系统介导的糖转运。酶I的固有荧光。
J Biol Chem. 1987 Nov 25;262(33):15900-7.
10
Reduced bovine pancreatic trypsin inhibitor has a compact structure.还原型牛胰蛋白酶抑制剂具有紧密的结构。
Biochemistry. 1988 Dec 13;27(25):8889-93. doi: 10.1021/bi00425a003.

Approaches to teaching fluorescence spectroscopy.

作者信息

Royer C A

机构信息

University of Wisconsin-Madison School of Pharmacy 53706, USA.

出版信息

Biophys J. 1995 Mar;68(3):1191-5. doi: 10.1016/S0006-3495(95)80295-X.

DOI:10.1016/S0006-3495(95)80295-X
PMID:7756538
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1281842/
Abstract
摘要