Wisnewski A V, Olds G R, Kresina T F
Department of Molecular, Cell Biology and Biochemistry, Brown University, Providence RI, USA.
Hum Antibodies Hybridomas. 1994;5(3-4):178-82.
Hybridomas secreting human monoclonal antibodies (hMAb) were derived from Epstein Barr Virus (EBV) transformed lymphocytes of a patient with acute Schistosoma japonicum infection. Three IgG1 hMAb SJ-D, SJ-E, and SJ-F bind soluble egg antigens (SEA) as determined by ELISA. These hMAb exhibit identical western blot profiles, recognizing an epitope(s) of multiple antigens with apparent molecular weights between 42 and 75 kDa. Serological analysis of these hMAb revealed a heterogeneity in their expression of a specific human S. japonicum anti-SEA associated cross reactive idiotype designated Hu SJ-CRIM. The differential expression of idiotypy by these hMAb correlates with immunosuppression of blastogenesis of lymphocytes from schistosomiasis patients. The level of suppression mediated by hMAb expressing high levels of Hu SJ-CRIM ranged from 41% to 52% (p < 0.05) for antigen and 36% to 43% for mitogen. In contrast, hMAb SJ-D which expressed over two fold lower levels Hu SR-CRIM, on a per weight basis showed no suppressive immune function. The data show the heterogeneous expression of human idiotype associated with S. japonicum infection and the correlation of idiotype expression with immune function.
分泌人单克隆抗体（hMAb）的杂交瘤源自一名急性日本血吸虫感染患者的爱泼斯坦-巴尔病毒（EBV）转化淋巴细胞。通过ELISA测定，三种IgG1 hMAb SJ-D、SJ-E和SJ-F与可溶性虫卵抗原（SEA）结合。这些hMAb呈现相同的蛋白质印迹图谱，识别多种抗原的一个表位，其表观分子量在42至75 kDa之间。对这些hMAb的血清学分析显示，它们在一种特定的人日本血吸虫抗SEA相关交叉反应独特型（称为Hu SJ-CRIM）的表达上存在异质性。这些hMAb独特型的差异表达与血吸虫病患者淋巴细胞增殖的免疫抑制相关。表达高水平Hu SJ-CRIM的hMAb介导的抑制水平，抗原刺激时为41%至52%（p < 0.05），丝裂原刺激时为36%至43%。相比之下，每重量基础上表达的Hu SR-CRIM水平低两倍以上的hMAb SJ-D没有显示出抑制性免疫功能。数据表明与日本血吸虫感染相关的人独特型的异质性表达以及独特型表达与免疫功能的相关性。
