Kresina T F, Olds G R
Infect Immun. 1986 Jul;53(1):90-4. doi: 10.1128/iai.53.1.90-94.1986.
In this study the expression of a regulatory cross-reactive idiotype (SJ-CRIM), which is associated with anti-soluble egg antigen (SEA) molecules in murine Schistosoma japonicum infection, is described. Both humoral and cellular components of the immune response were analyzed during the course of infection with S. japonicum. In the humoral immune response, the content of SJ-CRIM decreases as the titer of anti-SEA antibody increases throughout infection. Quantitatively, values for serum ranged from 13.8 +/- 0.3 micrograms of SJ-CRIM, which binds anti-idiotypic antibody per ml of serum at 6 weeks postinfection, to 1.3 +/- 1.8 micrograms/ml at 30 weeks postinfection. Analysis of splenic cell subpopulations for expression of SJ-CRIM revealed that only splenic B cells expressed SJ-CRIM during acute infection (5 to 10 weeks postinfection). On the other hand, thymic cells with a high expression of the SJ-CRIM and Ly-1 marker were observed in acute infections up to 15 weeks postinfection. These data indicate that SJ-CRIM-bearing T cells are selectively localized in acute infection. In addition, the disappearance of expression of SJ-CRIM in serum and cells of chronically infected animals parallels the modulation of granulomatous inflammation and portal hypertension. Results of this study suggest that expression of SJ-CRIM on anti-SEA molecules could represent a marker for acute infection, while its disappearance from serum serves as a marker for modulation of disease.
本研究描述了一种调节性交叉反应独特型（SJ-CRIM）的表达，该独特型与日本血吸虫感染小鼠体内的抗可溶性虫卵抗原（SEA）分子相关。在日本血吸虫感染过程中，对免疫反应的体液和细胞成分进行了分析。在体液免疫反应中，随着感染过程中抗SEA抗体效价的升高，SJ-CRIM的含量降低。定量分析显示，血清中SJ-CRIM的值范围为：感染后6周时，每毫升血清中与抗独特型抗体结合的SJ-CRIM为13.8±0.3微克；感染后30周时，为1.3±1.8微克/毫升。对脾细胞亚群进行SJ-CRIM表达分析发现，在急性感染期（感染后5至10周），只有脾B细胞表达SJ-CRIM。另一方面，在感染后长达15周的急性感染中，观察到具有高SJ-CRIM和Ly-1标记表达的胸腺细胞。这些数据表明，携带SJ-CRIM的T细胞在急性感染中选择性定位。此外，慢性感染动物血清和细胞中SJ-CRIM表达的消失与肉芽肿性炎症和门静脉高压的调节平行。本研究结果表明，抗SEA分子上SJ-CRIM的表达可能代表急性感染的一个标志物，而其从血清中的消失则作为疾病调节的一个标志物。
