Nagatsuka Y, Hanazawa S, Yasuda T, Ono Y
Department of Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Hum Antibodies Hybridomas. 1994;5(3-4):183-6.
Oligoclonal B-lymphoblastoid cell lines (LCL) were blindly established by nonimmunized protocol from natural populations of two normal persons and 8 autoimmune disease patients using Epstein-Barr virus (EBV)-induced transformation. We systematically screened these LCLs on antibodies against a panel of glycolipids using liposome immune lysis assay (LILA). Eventually we found antibodies to 12 out of 15 compounds containing CTH, globoside, Forssman, paragloboside, CPH, sulfatide, NAGM3, NGGM3, i active glycolipid, GM1, GA1 and GA2 in 81 out of 950 LCLs from normal PBL, and antibodies to 2 out of 15 compounds containing sulfatide and CPH were also detected in 11 out of 430 LCLs from autoimmune disease patients. Unexpectedly, the antibody repertoire of LCLs from autoimmune disease patients was impoverished. The possibility of the EBV technique for production of various kinds of human monoclonal antibodies (MAbs) to glycolipid was shown.
通过爱泼斯坦-巴尔病毒（EBV）诱导转化，采用非免疫方案从两名正常人和8名自身免疫性疾病患者的自然群体中盲目建立了寡克隆B淋巴母细胞系（LCL）。我们使用脂质体免疫裂解试验（LILA），针对一组糖脂抗体对这些LCL进行了系统筛选。最终，我们在来自正常外周血淋巴细胞（PBL）的950个LCL中的81个中发现了针对15种含CTH、红细胞糖苷脂、福斯曼、异红细胞糖苷脂、CPH、硫脂、NAGM3、NGGM3、i活性糖脂、GM1、GA1和GA2化合物中12种的抗体，并且在来自自身免疫性疾病患者的430个LCL中的11个中也检测到了针对15种含硫脂和CPH化合物中2种的抗体。出乎意料的是，来自自身免疫性疾病患者的LCL的抗体库有所减少。结果表明了EBV技术用于生产各种针对糖脂的人单克隆抗体（MAb）的可能性。
