Koechli O R, Schaer G N, Sevin B U, Perras J P, Schenk V, Rodriguez M, Untch M, Steren A, Haller U
Department of Obstetrics & Gynecology, University of Zurich, Switzerland.
Anticancer Drugs. 1995 Feb;6(1):94-100. doi: 10.1097/00001813-199502000-00011.
This is the first report on the ATP cell viability assay as a chemosensitivity test system for gestational trophoblastic neoplasms (GTN). We obtained chemosensitivity profiles in two established trophoblastic cell lines and four fresh tumors. Ten drugs were tested in vitro in the two cell lines JAR and JEG-3. The IC50 values of the 10 chemotherapeutic agents tested were very similar for both cell lines. The three most active drugs in these cell lines were VP-16, paclitaxel and vincristine. This is the first report on the activity of paclitaxel in trophoblastic cell lines. We furthermore evaluated this assay for chemosensitivity testing in four fresh malignant GTN tumors: one placental site trophoblastic tumor, one chorocarcinoma and two invasive moles. The placental site trophoblastic tumor specimen revealed to be rather chemoresistant in vitro whereas the other three tumors were chemosensitive. From our cell line data we conclude that the ATP cell viability assay is a practicable assay for chemosensitivity testing of GTN cell lines and gives repeatable results. However, the value of this assay for fresh GTN chemosensitivity testing needs to be defined.
这是关于将ATP细胞活力测定作为妊娠滋养细胞肿瘤（GTN）化学敏感性测试系统的首篇报道。我们在两种已建立的滋养细胞系和四个新鲜肿瘤中获得了化学敏感性图谱。在JAR和JEG - 3这两种细胞系中对十种药物进行了体外测试。所测试的10种化疗药物的IC50值在两种细胞系中非常相似。在这些细胞系中活性最高的三种药物是依托泊苷、紫杉醇和长春新碱。这是关于紫杉醇在滋养细胞系中活性的首篇报道。此外，我们在四个新鲜的恶性GTN肿瘤中评估了该测定用于化学敏感性测试的情况：一个胎盘部位滋养细胞肿瘤、一个绒毛膜癌和两个侵蚀性葡萄胎。胎盘部位滋养细胞肿瘤标本在体外显示出相当的化疗耐药性，而其他三个肿瘤则具有化学敏感性。根据我们的细胞系数据，我们得出结论，ATP细胞活力测定是一种用于GTN细胞系化学敏感性测试的可行测定方法，并且能给出可重复的结果。然而，该测定在新鲜GTN化学敏感性测试中的价值尚需明确。
