Tay S S
Department of Anatomy, Faculty of Medicine, National University of Singapore.
Histol Histopathol. 1995 Jan;10(1):85-9.
NADPH-diaphorase activity was localized in pancreatic ganglia of the young chick. At 1 day posthatching, 60% of the neurons in the pancreatic ganglia were NADPH-diaphorase positive. In each neuron, the NADPH-diaphorase labelling was localized mainly in the cytoplasm of the cell body and its proximal processes, but not in the cell nucleus. There was a gradation in the labelling for the enzyme, with some neurons being heavily labelled while others were lightly to moderately labelled. At 7 days post-hatching, 100% of the pancreatic neurons showed NADPH-diaphorase activity and the average size of the NADPH-diaphorase positive neurons had also increased. By 14 days post-hatching, all the neurons present were heavily labelled for NADPH-diaphorase activity. Some of the labelled nerve processes traversed long distances and finally terminated on other ganglia as well as on the exocrine acinar or endocrine cells. It is concluded that this increase in NADPH-diaphorase/NOS activity in the pancreatic neurons is possibly correlated to the increase in modulation of neurotransmission in the young chick.
烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）活性定位于幼雏鸡的胰腺神经节。孵化后1天，胰腺神经节中60%的神经元烟酰胺腺嘌呤二核苷酸磷酸黄递酶呈阳性。在每个神经元中，烟酰胺腺嘌呤二核苷酸磷酸黄递酶标记主要定位于细胞体及其近端突起的细胞质中，而不在细胞核中。该酶的标记存在梯度差异，一些神经元标记强烈，而另一些则为轻度至中度标记。孵化后7天，100%的胰腺神经元呈现烟酰胺腺嘌呤二核苷酸磷酸黄递酶活性，且烟酰胺腺嘌呤二核苷酸磷酸黄递酶阳性神经元的平均大小也有所增加。到孵化后14天，所有现存神经元的烟酰胺腺嘌呤二核苷酸磷酸黄递酶活性标记均强烈。一些标记的神经突起穿过很长距离，最终终止于其他神经节以及外分泌腺泡或内分泌细胞上。结论是，胰腺神经元中烟酰胺腺嘌呤二核苷酸磷酸黄递酶/一氧化氮合酶（NOS）活性的这种增加可能与幼雏鸡神经传递调节的增加相关。
