Gehring T A, Cooper W M, Holder C L, Thompson H C
U.S. Food and Drug Administration, National Center for Toxicological Research, Division of Chemistry, Jefferson, AR 72079, USA.
J AOAC Int. 1995 Mar-Apr;78(2):307-9.
A liquid chromatographic method was developed for determination of the essential nutrient thiamine (vitamin B1) in rodent feed. Thiamine was extracted with hydrochloric acid, separated by reversed-phase liquid chromatography, derivatized postcolumn to thiochrome with potassium hydroxide and potassium ferricyanide, and detected by fluorescence. Excitation and emission wavelengths were 370 and 430 nm, respectively. Detector response was linear in the range of 2.58 to 15.5 ng of thiamine injected. Instrument detection limit was 5 pg of thiamine injected.
