Cutting J H, Kiessling W M, Bond F L, McCarron J E, Kreuzer K S, Hurlbut J A, Sofos J N
U.S. Food and Drug Administration, Denver District Laboratory, CO 80225-0087, USA.
J AOAC Int. 1995 May-Jun;78(3):663-7.
An electrophoretic method coupled with bioautography was developed for detection and identification of penicillin G, ampicillin, amoxicillin, cloxacillin, cephapirin, and ceftiofur residues in milk. The method uses a 2% agarose gel for electrophoresis, an overlay of PM indicator agar seeded with Bacillus stearothermophilus var. calidolactis, and incubation at 55 degrees C for 16-18 h. The new method separated and detected residues in milk at the levels of concern for the Food and Drug Administration (FDA) for penicillin G (5 ppb), cephapirin (20 ppb), and ceftiofur (50 ppb). The method also detected ampicillin, amoxicillin, and cloxacillin at 20, 30, and 30 ppb, respectively, but these levels are above those of concern for FDA (10 ppb).
开发了一种结合生物自显影的电泳方法，用于检测和鉴定牛奶中的青霉素G、氨苄青霉素、阿莫西林、氯唑西林、头孢匹林和头孢噻呋残留。该方法使用2%琼脂糖凝胶进行电泳，在接种嗜热脂肪芽孢杆菌热乳酸变种的PM指示琼脂上覆盖，并在55℃孵育16 - 18小时。新方法能够分离并检测出牛奶中达到美国食品药品监督管理局（FDA）关注水平的青霉素G（5 ppb）、头孢匹林（20 ppb）和头孢噻呋（50 ppb）的残留。该方法还分别检测出20、30和30 ppb的氨苄青霉素、阿莫西林和氯唑西林，但这些水平高于FDA关注的水平（10 ppb）。
