Leaves N I, Anderson E C, Toy S J
Public Health Laboratory Service Haemophilus Reference Laboratory, John Radcliffe Hospital, Oxford, England, UK.
Br J Biomed Sci. 1994 Dec;51(4):307-11.
Outer membrane protein profiling was used to assist in determining the identity of an isolate of Haemophilus spp. that was presumptively identified as non-capsulate Haemophilus influenzae biotype III. The possibility that this strain was in fact Haemophilus aegyptius was queried because of clinical information and the source of the isolate. Sodium dodecyl-sulphate polyacrylamide gel electrophoresis was used to establish the identity of the isolate as non-capsulate H. influenzae biotype III and no H. aegyptius. Generally, protein profiling compared very favourably with other standard tests for identifying H. aegyptius: the method was easily and rapidly performed and gave an unequivocal result.
外膜蛋白谱分析用于协助确定一株嗜血杆菌属分离株的身份，该分离株初步鉴定为非荚膜型流感嗜血杆菌生物型III。由于临床信息和分离株的来源，有人质疑该菌株实际上可能是埃及嗜血杆菌。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳确定该分离株为非荚膜型流感嗜血杆菌生物型III，而非埃及嗜血杆菌。一般来说，与其他鉴定埃及嗜血杆菌的标准试验相比，蛋白谱分析具有很大优势：该方法操作简便、快速，结果明确。
