Ventimiglia R, Mather P E, Jones B E, Lindsay R M
Regeneron Pharmaceuticals, Inc., Tarrytown, NY 10591, USA.
Eur J Neurosci. 1995 Feb 1;7(2):213-22. doi: 10.1111/j.1460-9568.1995.tb01057.x.
The neurotrophins, brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), neurotrophin 4/5 (NT-4/5) and nerve growth factor (NGF), were compared for their effects on the survival and differentiation of embryonic rat striatal neurons grown in low-density cultures. Treatment with BDNF for 8 days resulted in a 40% increase in overall neuronal survival, a 3- to 5-fold increase in the number of calbindin-immunoreactive neurons, and an 80% increase in GABA-positive neurons. Treatment with NT-3 or NT-4/5 produced a 2- to 3-fold increase in the number of calbindin-positive neurons and an increase in GABA-positive cell number similar to that induced by BDNF, BDNF treatment produced a striking morphological differentiation of striatal GABAergic neurons, which was characterized by a doubling of the number of neurite branch points, the total area of aborization and the perikaryal area compared to control cultures. All three of these factors increased high-affinity GABA uptake 2-fold. NGF had no effect on any of the parameters examined. Our results show that BDNF, NT-3 and NT-4/5 promote the survival and/or differentiation of calbindin-immunopositive and GABAergic striatal neurons.
对神经营养因子、脑源性神经营养因子（BDNF）、神经营养因子-3（NT-3）、神经营养因子4/5（NT-4/5）和神经生长因子（NGF）在低密度培养条件下对胚胎大鼠纹状体神经元存活及分化的影响进行了比较。用BDNF处理8天导致总体神经元存活率提高40%，钙结合蛋白免疫反应性神经元数量增加3至5倍，GABA阳性神经元数量增加80%。用NT-3或NT-4/5处理使钙结合蛋白阳性神经元数量增加2至3倍，GABA阳性细胞数量增加程度与BDNF诱导的相似。BDNF处理使纹状体GABA能神经元产生显著的形态分化，其特征是与对照培养相比，神经突分支点数量、树突总面积和胞体面积增加了一倍。所有这三种因子使高亲和力GABA摄取增加了2倍。NGF对所检测的任何参数均无影响。我们的结果表明，BDNF、NT-3和NT-4/5促进了钙结合蛋白免疫阳性和GABA能纹状体神经元的存活和/或分化。
