Wonisch W, Schaur R J, Bilinski T, Esterbauer H
Institute of Biochemistry, University of Graz, Austria.
Cell Biochem Funct. 1995 Jun;13(2):91-8. doi: 10.1002/cbf.290130205.
The effect of pretreatment with aldehydes on the subsequent colony forming efficiency (CFE) of Saccharomyces cerevisiae was investigated. All 21 aldehydes tested inhibited CFE in a dose-dependent manner. The effective doses, however, differed markedly from 300 mM to 0.07 mM depending on the functional groups and chain length of the aldehydes. Amongst the nine representatives of n-alkanals, formaldehyde was the most potent inhibitor, reducing CFE to 50 per cent at a dose of 0.3 mM (IC50). In the series of 2-trans-alkenals, acrolein was most effective with an IC50 of 0.08 mM and amongst the 4-hydroxy 2-trans-alkenals, 4-hydroxynonenal was most effective with IC50 of 0.07 mM. In general, effectiveness decreased in the order: 4-hydroxyalkenals > 2-alkenals >> n-alkenals. It is proposed that S. cerevisiae is a promising target cell to elucidate further the molecular mechanisms by which aldehydes, particularly the lipid peroxidation product 4-hydroxynonenal, inhibits cell proliferation.
研究了用醛进行预处理对酿酒酵母后续集落形成效率（CFE）的影响。所测试的21种醛均以剂量依赖方式抑制CFE。然而，有效剂量根据醛的官能团和链长不同，在300 mM至0.07 mM之间有显著差异。在九种正构烷醛代表物中，甲醛是最有效的抑制剂，在0.3 mM剂量（IC50）时将CFE降低至50%。在2-反式烯醛系列中，丙烯醛最有效，IC50为0.08 mM；在4-羟基-2-反式烯醛中，4-羟基壬烯醛最有效，IC50为0.07 mM。一般来说，有效性按以下顺序降低：4-羟基烯醛＞2-烯醛＞＞正构烯醛。有人提出，酿酒酵母是一个很有前景的靶细胞，可用于进一步阐明醛类，特别是脂质过氧化产物4-羟基壬烯醛抑制细胞增殖的分子机制。
