Esterbauer H, Zollner H
Institute of Biochemistry, University of Graz, Austria.
Free Radic Biol Med. 1989;7(2):197-203. doi: 10.1016/0891-5849(89)90015-4.
A complex pattern of aldehydes (alkanals, 2-alkenals, 2,4-alkadienals, 4-hydroxyalkenals) is generated by peroxidizing biological samples. Several methods based on HPLC or GC-MS have been developed to qualitatively and quantitatively measure the aldehydes in tissues, cells and cell fractions exposed to various pro-oxidative stimuli. 4-Hydroxynonenal, hexanal and propanal are, besides malonaldehyde, the most abundant aldehydes formed. The high sensitivity of the methods also allows the measurement of physiological aldehyde levels in plasma or low density lipoproteins and this could be of great importance for in vivo studies.
通过对生物样品进行过氧化反应会生成醛类（链烷醛、2-链烯醛、2,4-链二烯醛、4-羟基链烯醛）的复杂模式。已经开发了几种基于高效液相色谱（HPLC）或气相色谱-质谱联用（GC-MS）的方法，用于定性和定量测量暴露于各种促氧化刺激的组织、细胞和细胞组分中的醛类。除丙二醛外，4-羟基壬醛、己醛和丙醛是生成的最丰富的醛类。这些方法的高灵敏度还允许测量血浆或低密度脂蛋白中的生理醛水平，这对于体内研究可能非常重要。
