用于检测肠侵袭性大肠杆菌和毒性志贺氏菌菌株的改良酶联免疫吸附测定法。
Modified enzyme-linked immunosorbent assay for detecting enteroinvasive Escherichia coli and virulent Shigella strains.
作者信息
Pál T, Pácsa A S, Emödy L, Vörös S, Sélley E
出版信息
J Clin Microbiol. 1985 Mar;21(3):415-8. doi: 10.1128/jcm.21.3.415-418.1985.
Abstract
Immune sera were produced in rabbits with living cells of an enteroinvasive O143 strain of Escherichia coli. To remove O and K antibodies, sera were absorbed with an avirulent derivative of the same strain. In the enzyme-linked immunosorbent assay, absorbed sera reacted specifically with only virulent Shigella strains and enteroinvasive E. coli strains of different geographical origin, regardless of species or serogroups. The investigation of 83 strains indicated complete agreement between enzyme-linked immunosorbent assay results and those of the keratoconjunctivitis test. It is assumed that the absorbed immune sera reacted with a possible virulence marker antigen. This inexpensive and simple method provides an alternative to other virulence tests. It has a definite advantage for screening large number of isolates within 24 h.
摘要
用肠侵袭性大肠杆菌O143菌株的活细胞在兔体内制备免疫血清。为去除O和K抗体，血清用同一菌株的无毒衍生物进行吸收。在酶联免疫吸附试验中，吸收后的血清仅与不同地理来源的有毒志贺氏菌菌株和肠侵袭性大肠杆菌菌株发生特异性反应，而与物种或血清群无关。对83株菌株的研究表明，酶联免疫吸附试验结果与角膜结膜炎试验结果完全一致。推测吸收后的免疫血清与一种可能的毒力标记抗原发生了反应。这种廉价且简单的方法为其他毒力试验提供了一种替代方法。它在24小时内筛选大量分离株方面具有明显优势。
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