Rodriguez M C, Zamudio F, Torres J A, Gonzalez-Ceron L, Possani L D, Rodriguez M H
Centro de Investigación de Paludismo, Ministry of Health, Tapachula, Chiapas, Mexico.
Exp Parasitol. 1995 Jun;80(4):596-604. doi: 10.1006/expr.1995.1075.
The effect of a synthetic cecropin-like peptide, Shiva-3, on in vitro ookinete development and on the early sporogonic stages of Plasmodium berghei in the midgut of Anopheles albimanus was investigated. Peptide concentrations of 75 and 100 microM were effective (P < 0.05) in reducing ookinete production and the number of infected mosquitoes in almost all experiments. These peptide concentrations in the midgut were not toxic for the survival of the mosquitoes. Complete inhibition was obtained if 100 microM Shiva-3 was applied in the first 8 hr of parasite development. The deleterious effect of the peptide on the parasite was effective after exposure for as short as 50 sec and the permanence of free peptide in the mosquito midgut was estimated to be of a minimum of 5 min. These observations indicate the possibility of using Shiva-like peptide genes to engineering malaria-resistant vectors as an alternative in malaria control strategies.
研究了一种合成的类天蚕素肽Shiva-3对体外动合子发育以及白纹按蚊中肠内伯氏疟原虫早期孢子生殖阶段的影响。在几乎所有实验中，75和100微摩尔浓度的肽在减少动合子产生和感染蚊子数量方面有效（P<0.05）。中肠内的这些肽浓度对蚊子的存活无毒。如果在寄生虫发育的前8小时应用100微摩尔的Shiva-3，可实现完全抑制。该肽对寄生虫的有害作用在暴露仅50秒后即有效，且估计游离肽在蚊子中肠内的持续时间至少为5分钟。这些观察结果表明，利用类Shiva肽基因构建抗疟蚊媒作为疟疾控制策略的一种替代方法具有可能性。
