Parent-Massin D, Thouvenot D
Laboratoire de Microbiologie et Biochimie, Ecole Supérieure de Microbiologie et Sécurite Alimentaire, ISAMOR, Technopôle Brest-Iroise, Plouzane, France.
Food Addit Contam. 1995 Jan-Feb;12(1):41-9. doi: 10.1080/02652039509374277.
The fusarial toxicosis induced by trichothecenes is characterized by common syndromes such as vomiting, inflammation, haemorrhages, diarrhoea and haematological changes. Subchronic ingestion of trichothecenes causes a decrease in circulating white cells. This leukopenic change of animals is reported as a characteristic feature in the best known human disorder: Alimentary Toxic Aleukia (ATA). The aim of the present study was to evaluate whether the haematologic disorders imputed to trichothecenes were a result of myelotoxicity by investigating in an in vitro model. Rat haematopoietic progenitors, Colony Forming Units-Granulocytes and Macrophages (CFU-GM), were cultured in the presence of several concentrations of four trichothecenes; T-2 toxin, HT-2 toxin, diacetoxyscirpenol (DAS) and deoxynivalenol (DON). All these trichothecenes were cytotoxic to rat haematopoietic progenitor cells. It is concluded that haematological disorders observed during trichothecene intoxication of animals are caused by the destruction of haematopoietic progenitors such as CFU-GM cells.
由单端孢霉烯族毒素引起的镰刀菌中毒具有呕吐、炎症、出血、腹泻和血液学变化等常见症状。亚慢性摄入单端孢霉烯族毒素会导致循环白细胞减少。动物的这种白细胞减少变化在最著名的人类疾病：食源性中毒性白细胞缺乏症（ATA）中被报道为一个特征。本研究的目的是通过在体外模型中进行研究，评估归因于单端孢霉烯族毒素的血液学紊乱是否是骨髓毒性的结果。将大鼠造血祖细胞，即粒细胞和巨噬细胞集落形成单位（CFU-GM），在几种浓度的四种单端孢霉烯族毒素；T-2毒素、HT-2毒素、二醋酸藨草镰刀菌烯醇（DAS）和脱氧雪腐镰刀菌烯醇（DON）存在的情况下进行培养。所有这些单端孢霉烯族毒素对大鼠造血祖细胞均具有细胞毒性。得出的结论是，在动物单端孢霉烯族毒素中毒期间观察到血液学紊乱是由造血祖细胞如CFU-GM细胞的破坏引起的。
