二乙酰氧基雪腐镰刀菌烯醇（DAS）对人和大鼠粒单核祖细胞的体外作用。

In vitro effects of diacetoxyscirpenol (DAS) on human and rat granulo-monocytic progenitors.

作者信息

Lautraite S, Rio B, Guinard J, Parent-Massin D

机构信息

Laboratoire de Microbiologie et de Sécurité Alimentaire, Ecole Supérieure de Microbiologie et Sécurité Alimentaire de Brest, ISAMOR, Plouzané, France.

出版信息

Mycopathologia. 1997;140(1):59-64. doi: 10.1023/a:1006833606209.

DOI:10.1023/a:1006833606209

PMID:9608725

Abstract

Diacetoxyscirpenol (DAS) is a trichothecene mycotoxin produced by various species of fungi. Trichothecenes are known as major contaminants of cereals and cereal-containing foods. DAS has been detected in agricultural products worldwide and persists in products after processing. In human as well as in animals, DAS consumption has been shown to induce haematological disorders (neutropenia, aplastic anemia). Granulo-monocytic progenitors (CFU-GM) from human umbilical cord blood and rat bone marrow have been cultured in the presence of DAS (from 10(-8) M to 5 x 10(-10) M) for 14 days. Study of concentration and effect relationships have shown a sharp effect of DAS on rat CFU-GM between 10(-7) M and 10(-8) M, while human CFU-GM are able to grow in the presence of 10(-8) M of the toxin. IC50 values on day 14 are respectively, 7.6 x 10(-9) M for human CFU-GM and 6.2 x 10(-9) M for rat CFU-GM.

摘要

二乙酰氧基雪腐镰刀菌烯醇（DAS）是由多种真菌产生的单端孢霉烯族毒素。单端孢霉烯族毒素是谷物及含谷物食品的主要污染物。DAS在世界各地的农产品中均有检出，且在加工后的产品中依然存在。在人类和动物中，摄入DAS已被证明会引发血液系统疾病（中性粒细胞减少症、再生障碍性贫血）。人脐带血和大鼠骨髓中的粒-单核祖细胞（CFU-GM）已在含有DAS（浓度从10^(-8) M至5×10^(-10) M）的环境中培养了14天。浓度-效应关系研究表明，在10^(-7) M至10^(-8) M之间，DAS对大鼠CFU-GM有显著影响，而人CFU-GM在10^(-8) M毒素存在的情况下仍能生长。第14天的IC50值，人CFU-GM为7.6×10^(-9) M，大鼠CFU-GM为6.2×10^(-9) M。