Hayes N V, Holmes F E, Grantham J, Baines A J
Research School of Biosciences, University of Kent, Canterbury, U.K.
Biochem Soc Trans. 1995 Feb;23(1):54-8. doi: 10.1042/bst0230054.
A60 is a 60 kDa protein which is associated with the axonal cortical cytoskeleton in adult central nervous system neurons. It was originally defined by the use of a monoclonal antibody which showed that it was closely associated with the cytoplasic face of axonal plasma membranes. A60 appears to be neuron-specific and biochemical studies show that it is tightly bound to brain membranes. Affinity chromatography has revealed that A60 interacts with brain spectrin but not with erythrocyte spectrin. As erythrocyte spectrin is closely related to the isotype of spectrin that is localized in dendrites this raises the possibility that A60 is restricted to axons by interaction with the isotype of spectrin that is found in axons. During post-natal cerebellar development (days 1-13) A60 is initially located in the perikarya of precursor Purkinje cells and is then localized in the initial dendrites of these cells as well as in the white matter. In contrast, in the adult cerebellum the location of A60 is exclusively axonal. These data indicate that A60 has a spectrin-binding activity in the adult axonal membrane skeleton which is only required after initial axon growth has occurred. A60 is transiently expressed during embryonic and post-natal development of rat dorsal root ganglia (DRG). It is located in the large light DRG cells but is essentially absent from the small dark DRG cells.
A60是一种60 kDa的蛋白质，与成年中枢神经系统神经元的轴突皮质细胞骨架相关。它最初是通过使用一种单克隆抗体定义的，该抗体表明它与轴突质膜的细胞质面密切相关。A60似乎具有神经元特异性，生化研究表明它与脑膜紧密结合。亲和层析显示A60与脑血影蛋白相互作用，但不与红细胞血影蛋白相互作用。由于红细胞血影蛋白与定位于树突中的血影蛋白同种型密切相关，这就增加了A60通过与轴突中发现的血影蛋白同种型相互作用而局限于轴突的可能性。在出生后小脑发育（第1 - 13天）期间，A60最初位于前体浦肯野细胞的胞体中，然后定位于这些细胞的初始树突以及白质中。相比之下，在成年小脑中，A60仅位于轴突中。这些数据表明A60在成年轴突膜骨架中具有血影蛋白结合活性，而这种活性仅在轴突初始生长发生后才需要。A60在大鼠背根神经节（DRG）的胚胎期和出生后发育过程中短暂表达。它位于大的浅色DRG细胞中，但在小的深色DRG细胞中基本不存在。
