Fukuda M N, Sato T, Nakayama J, Klier G, Mikami M, Aoki D, Nozawa S
La Jolla Cancer Research Foundation, California 92037, USA.
Genes Dev. 1995 May 15;9(10):1199-210. doi: 10.1101/gad.9.10.1199.
Two human epithelial cell lines, trophoblastic teratocarcinoma HT-H and endometrial adenocarcinoma SNG-M cells, adhere to each other at their respective apical cell surfaces in a divalent cation-independent manner. Two novel molecules responsible for the adhesion between these two cell types were identified by expression cDNA cloning. One, named trophinin, is an intrinsic membrane protein and mediates homophilic self-binding. Another, named tastin, is a cytoplasmic protein and is necessary for trophinin to function as a cell adhesion molecule. Trophinin and tastin appear to be associated with the cytoskeleton in HT-H and SNG-M cells. These molecules are normally not expressed in various types of human cells in tissues, with the exception of macrophages. Strong expression of these molecules was detected in the trophectoderm surface of monkey blastocyst. These molecules are also expressed in human endometrial surface epithelium on day 16/17 at the early secretory phase of human endometrium, the time consistent with that expected for the "implantation window."
两种人类上皮细胞系，滋养层畸胎癌HT-H细胞和子宫内膜腺癌SNG-M细胞，以不依赖二价阳离子的方式在各自的顶端细胞表面相互黏附。通过表达性cDNA克隆鉴定出两种负责这两种细胞类型间黏附的新分子。一种名为trophinin，是一种内在膜蛋白，介导同嗜性自我结合。另一种名为tastin，是一种细胞质蛋白，是trophinin发挥细胞黏附分子功能所必需的。Trophinin和tastin似乎与HT-H和SNG-M细胞中的细胞骨架相关。除巨噬细胞外，这些分子通常不在组织中的各种人类细胞类型中表达。在猴胚泡的滋养外胚层表面检测到这些分子的强表达。在人类子宫内膜早期分泌期的第16/17天，这些分子也在人类子宫内膜表面上皮中表达，这一时期与预期的“着床窗”时间一致。
