海鞘同源框基因的克隆为原始脊索动物基因簇提供了证据。

Cloning of ascidian homeobox genes provides evidence for a primordial chordate cluster.

作者信息

Di Gregorio A, Spagnuolo A, Ristoratore F, Pischetola M, Aniello F, Branno M, Cariello L, Di Lauro R

机构信息

Laboratory of Biochemistry and Molecular Biology, Stazione Zoologica Anton Dohrn, Naples, Italy.

出版信息

Gene. 1995 Apr 24;156(2):253-7. doi: 10.1016/0378-1119(95)00035-5.

DOI:10.1016/0378-1119(95)00035-5

PMID:7758964

Abstract

In order to isolate genes important in controlling embryonic development in Tunicates, a genomic library from the ascidian Ciona intestinalis was screened with a degenerate oligodeoxyribonucleotide encoding the third helix of Antennapedia-type homeoboxes. Fourteen C. intestinalis homeobox genes, corresponding to several classes of homeodomains, have been identified. Five of the isolated homeoboxes show their highest homology to members of the Vertebrate HOX clusters. mRNAs for two of the isolated homeoboxes are present in unfertilized C. intestinalis eggs.

摘要

为了分离在控制被囊动物胚胎发育中起重要作用的基因，用编码触角足型同源异型框第三螺旋的简并寡脱氧核糖核苷酸筛选了来自海鞘肠鳃纲动物的基因组文库。已经鉴定出14个与几类同源结构域相对应的肠鳃纲动物同源异型框基因基因。分离出的5个同源异型框与脊椎动物HOX簇的成员具有最高的同源性。未受精的肠鳃纲动物卵中存在两个分离出的同源异型框的mRNA。