Ward R J, Monesi N, Arni R K, Larson R E, Paço-Larson M L
Departamento de Fisica, IBILCE-UNESP, São José do Rio Preto-SP, Brasil.
Gene. 1995 Apr 24;156(2):305-6. doi: 10.1016/0378-1119(95)00099-r.
With the aim of further understanding the structure/function relationships in the membrane-damaging activity of the Lys49 phospholipase A2 (Lys49-PLA2) sub-family, we used PCR (polymerase chain reaction) on total venom gland cDNAs from Bothrops jararacussu with degenerate oligodeoxyribonucleotides encoding the N- and C-termini of myotoxin II, a Lys49-PLA2 from Bothrops asper. A 350-bp cDNA coding for bothropstoxin I (BtxtxI) was amplified. Sequencing of the amplified fragment shows that BtxtxI has a Lys49, and comparison with the known structure of myotoxin II showed that the amino acids involved in the formation of a novel dimeric structure in this protein were also conserved.
为了进一步了解Lys49磷脂酶A2（Lys49-PLA2）亚家族膜损伤活性中的结构/功能关系，我们使用聚合酶链反应（PCR），以编码来自三色矛头蝮（Bothrops asper）的肌毒素II（一种Lys49-PLA2）N端和C端的简并寡脱氧核糖核苷酸，对巴西矛头蝮（Bothrops jararacussu）全毒腺cDNA进行扩增。扩增出一条编码矛头蝮毒素I（BtxtxI）的350 bp cDNA。对扩增片段的测序表明，BtxtxI含有一个Lys49，并且与肌毒素II的已知结构比较显示，该蛋白中形成新型二聚体结构所涉及的氨基酸也保守。
