Moura-da-Silva A M, Paine M J, Diniz M R, Theakston R D, Crampton J M
Wolfson Unit of Molecular Genetics, Liverpool School of Tropical Medicine, UK.
J Mol Evol. 1995 Aug;41(2):174-9. doi: 10.1007/BF00170670.
The sequence coding for a snake venom phospholipase A2 (PLA2), BJUPLA2, has been cloned from a Bothrops jararacussu venom gland cDNA library. The cDNA sequence predicts a precursor containing a 16-residue signal peptide followed by a molecule of 122 amino acid residues with a strong sequence similarity to group II snake venom PLA2's. A striking feature of the cDNA is the high sequence conservation of the 5' and 3' untranslated regions in cDNAs coding for PLA2's from a number of viper species. The greatest sequence variation was observed between the regions coding for the mature proteins, with most substitutions occurring in nonsynonymous sites. The phylogenetic tree constructed by alignment of the amino acid sequence of BJUPLA2 with group II PLA2's in general groups them according to current taxonomical divisions and/or functional activity. It also suggests that gene duplications may have occurred at a number of different points during the evolution of snake venom group II PLA2's.
已从巴西矛头蝮蛇毒腺cDNA文库中克隆出编码一种蛇毒磷脂酶A2（PLA2）即BJUPLA2的序列。该cDNA序列预测其前体包含一个16个残基的信号肽，其后是一个由122个氨基酸残基组成的分子，与II类蛇毒PLA2具有高度的序列相似性。该cDNA的一个显著特征是，在编码来自多种蝰蛇物种PLA2的cDNA中，5'和3'非翻译区具有高度的序列保守性。在编码成熟蛋白的区域之间观察到最大的序列变异，大多数替换发生在非同义位点。通过将BJUPLA2的氨基酸序列与II类PLA2进行比对构建的系统发育树，总体上根据当前的分类划分和/或功能活性对它们进行了分组。这也表明在蛇毒II类PLA2的进化过程中，可能在多个不同点发生了基因复制。
