Fuchs M, Gerlach J, Encke J, Unger J, Smith M, Neuhaus P, Riedel E
Klinikum Rudolf Virchow, Freie Universität, Berlin, Germany.
Int J Artif Organs. 1994 Dec;17(12):663-9.
The amino acid patterns of medium perfusate in a liver cell bioreactor developed for a hybrid liver support system have been measured. There were considerable changes in the concentrations of glutamic acid, glutamine, alanine, arginine, ornithine and branched chain amino acids during the first 10 days which is indicative of dynamic cellular metabolism. From day 15, steady state conditions of nitrogen metabolism are reflected by stable amino acid turnover. Monitoring of urea, K+, and P-450 activity suggests that hepatocytes have switched to a stable protein synthesis with a general amino acid uptake and keto acid release following cell volume increase.
已对为混合肝支持系统开发的肝细胞生物反应器中培养基灌注液的氨基酸模式进行了测量。在最初的10天里，谷氨酸、谷氨酰胺、丙氨酸、精氨酸、鸟氨酸和支链氨基酸的浓度发生了显著变化，这表明细胞代谢活跃。从第15天开始，稳定的氨基酸周转率反映了氮代谢的稳态。对尿素、钾离子和P - 450活性的监测表明，随着细胞体积增加，肝细胞已转向稳定的蛋白质合成，伴有一般氨基酸摄取和酮酸释放。
