Jonas S, John M, Boese-Landgraf J, Häring R, Prevost G, Thomas F, Rosewicz S, Riecken E O, Wiedenmann B, Neuhaus P
Freie Universität Berlin, Universitätsklinikum Rudolf Virchow, Department of Surgery, Germany.
Langenbecks Arch Chir. 1995;380(2):90-5. doi: 10.1007/BF00186414.
Somatostatin receptor scintigraphy (SRS) is positive in approximately 80% of all patients who have been found to have neuroendocrine (NE) gastroenteropancreatic (GEP) tumors. The reasons for negative results are unclear. The aim of the present study was identification of the specific somatostatin receptor (SSTR) subtypes that are responsible for the in vivo binding of the widely used somatostatin (SST) analogues octreotide and lanreotide in human neuroendocrine gastroenteropancreatic tumors. Ten patients were subjected to SRS with radiolabeled octreotide. Following surgical resection, tumor tissues were analyzed for SSTR subtype mRNA expression by the reverse transcription-polymerase chain reaction (RT-PCR). In addition, SSTR subtype transcripts were investigated by Northern blot analysis and RT-PCR in neuroendocrine tumor cell lines. Expression of SSTR at the protein level was studied by chemical cross-linking experiments. Three patients were negative by SRS. However, RT-PCR revealed most prominently SSTR 2 expression in all tumor specimens. In addition, all tumor tissues analyzed by chemical crosslinking exhibited SST-14 binding sites, indicating that at least some NE tumors were false-negative on SRS.
生长抑素受体闪烁扫描术（SRS）在所有已发现患有神经内分泌（NE）胃肠胰（GEP）肿瘤的患者中，约80%呈阳性。结果为阴性的原因尚不清楚。本研究的目的是确定在人类神经内分泌胃肠胰肿瘤中，负责广泛使用的生长抑素（SST）类似物奥曲肽和兰瑞肽体内结合的特定生长抑素受体（SSTR）亚型。10例患者接受了放射性标记奥曲肽的SRS检查。手术切除后，通过逆转录聚合酶链反应（RT-PCR）分析肿瘤组织中SSTR亚型mRNA的表达。此外，通过Northern印迹分析和RT-PCR研究神经内分泌肿瘤细胞系中的SSTR亚型转录本。通过化学交联实验研究SSTR在蛋白质水平的表达。3例患者SRS检查结果为阴性。然而，RT-PCR显示所有肿瘤标本中最显著的是SSTR 2表达。此外，通过化学交联分析的所有肿瘤组织均显示有SST-14结合位点，表明至少一些NE肿瘤在SRS检查中呈假阴性。
