Konnova S A, Skvortsov I M, Makarov O E, Ignatov V V
Mikrobiologiia. 1994 Nov-Dec;63(6):1020-30.
Azospirillum brasilense strains Sp7, Sp7 (S-form), Sp107, Sp245, Sp246, S17, and 15 when cultivated in a liquid synthetic malate medium up to the end of the logarithmic phase of growth were shown to produce at least two complex polysaccharide-containing components. The components were arbitrarily called lipopolysaccharide-protein complex (LPPC) and polysaccharide-lipid complex (PSLC). The LPPCs and PSLCs from the strains Sp7, Sp107, Sp245, Sp246, and S17 were shown to interact with a wheat germ agglutinin (WGA). From PSLCs, acidic polysaccharides (PS) were isolated and their specific rotation, molecular masses, affinity for WGA, and monosaccharide composition were determined. The PSs of all strains contained rhamnose, galacturonic acid, and galactose (except the strain Sp246), and glucosamine (except the strain Sp7 (S-form)), while the PSs of the strains Sp7 and S17 also contained fucose and mannose, respectively. It is suggested that LPPCs and PSLCs may be involved in the process of interaction of azospirilla with wheat root surfaces, and that PSsare probably active parts of the LPPCs and PSLCs. The ability of LPPCs and PSs to interact with Congo red in aqueous solutions was investigated.
当巴西固氮螺菌菌株Sp7、Sp7（S型）、Sp107、Sp245、Sp246、S17和15在液体合成苹果酸盐培养基中培养至对数生长期结束时，显示它们产生至少两种含复合多糖的组分。这些组分被随意地称为脂多糖-蛋白质复合物（LPPC）和多糖-脂质复合物（PSLC）。已证明来自菌株Sp7、Sp107、Sp245、Sp246和S17的LPPC和PSLC与麦胚凝集素（WGA）相互作用。从PSLC中分离出酸性多糖（PS），并测定了它们的比旋度、分子量、对WGA的亲和力和单糖组成。所有菌株的PS都含有鼠李糖、半乳糖醛酸和半乳糖（Sp246菌株除外），以及氨基葡萄糖（Sp7（S型）菌株除外），而Sp7和S17菌株的PS还分别含有岩藻糖和甘露糖。有人提出，LPPC和PSLC可能参与固氮螺菌与小麦根表面的相互作用过程，并且PS可能是LPPC和PSLC的活性部分。研究了LPPC和PS在水溶液中与刚果红相互作用的能力。
