Hanes D E, Koch W H, Miliotis M D, Lampel K A
Division of Virulence Assessment, Food and Drug Administration, Washington, DC 20204, USA.
Mol Cell Probes. 1995 Feb;9(1):9-18. doi: 10.1016/s0890-8508(95)90917-6.
Salmonellosis is the most frequently reported foodborne illness in the United States, with Salmonella enteritidis being the leading cause of these outbreaks. Nucleotide sequence comparisons of the Salmonella plasmid virulence (spv) genes of S. enteritidis with those of S. typhimurium and S. dublin have revealed that a single base-pair change unique to S. enteritidis is present in the spvA gene. An 18-base synthetic oligonucleotide probe (SE-probe) that is completely homologous to the spvA gene of S. enteritidis but which has one base pair mismatch with other salmonellae was shown to be specific for S. enteritidis. In colony hybridization blots, 129 isolates of S. enteritidis, 29 other species of Salmonella, and 17 non-Salmonella spp. were tested with the SE-probe. The SE-probe hybridized with 96% of the S. enteritidis strains tested but did not react with the other Salmonella or non-Salmonella strains. These data suggest that the SE-probe can be used in a specific and rapid detection assay for S. enteritidis.
沙门氏菌病是美国报告最多的食源性疾病，肠炎沙门氏菌是这些疫情爆发的主要原因。肠炎沙门氏菌的沙门氏菌质粒毒力（spv）基因与鼠伤寒沙门氏菌和都柏林沙门氏菌的核苷酸序列比较显示，spvA基因中存在肠炎沙门氏菌特有的单个碱基对变化。一种与肠炎沙门氏菌的spvA基因完全同源但与其他沙门氏菌有一个碱基对错配的18碱基合成寡核苷酸探针（SE探针）被证明对肠炎沙门氏菌具有特异性。在菌落杂交印迹中，用SE探针检测了129株肠炎沙门氏菌、29株其他沙门氏菌和17株非沙门氏菌属。SE探针与96%的受试肠炎沙门氏菌菌株杂交，但不与其他沙门氏菌或非沙门氏菌菌株反应。这些数据表明，SE探针可用于肠炎沙门氏菌的特异性快速检测试验。
