Inuo G, Akao N, Kohsaka H, Saito I, Miyasaka N, Fujita K
Department of Medical Zoology, School of Medicine, Tokyo Medical and Dental University, Japan.
Parasite Immunol. 1995 Feb;17(2):77-84. doi: 10.1111/j.1365-3024.1995.tb00969.x.
The proliferative response of human peripheral blood mononuclear cells (PBMC) from healthy donors to Toxocara canis adult worm antigens (TcA) was examined. PBMC from all donors examined (n = 7) strongly responded to TcA in a dose-dependent fashion after six days of culture, irrespective of their serological reactivity. In contrast, cord blood mononuclear cells did not react to TcA. The proliferation of PBMC in response to TcA was completely inhibited by anti-HLA-DR antibody. Purified CD4+ T cells reconstituted with autologous irradiated antigen presenting cells (APC) vigorously proliferated in response to TcA, but this was abrogated by pretreatment of APC with paraformaldehyde. Significant IL-2, IL-3, IL-4, IL-5 and IFN-gamma mRNA expression was detected in PBMC stimulated with TcA, with expression peaking at 72 h after stimulation. IL-1 beta, IL-6, IL-10 and GM-CSF mRNA expression was also upregulated, peaking at 24 h after stimulation. Taken together, these results suggest that adult T. canis-derived antigens have the ability to activate human PBMC as conventional antigens, possibly due to their cross-reactivity, which may be involved in the host defence against helminth infection.
检测了健康供体的人外周血单个核细胞（PBMC）对犬弓首蛔虫成虫抗原（TcA）的增殖反应。在培养6天后，所有检测的供体（n = 7）的PBMC均以剂量依赖方式对TcA产生强烈反应，与它们的血清学反应性无关。相比之下，脐血单个核细胞对TcA无反应。PBMC对TcA的增殖反应被抗HLA-DR抗体完全抑制。用自体照射的抗原呈递细胞（APC）重构的纯化CD4 + T细胞对TcA有强烈增殖反应，但用多聚甲醛预处理APC可消除这种反应。在用TcA刺激的PBMC中检测到显著的IL-2、IL-3、IL-4、IL-5和IFN-γ mRNA表达，刺激后72小时表达达到峰值。IL-1β、IL-6、IL-10和GM-CSF mRNA表达也上调，刺激后24小时达到峰值。综上所述，这些结果表明犬弓首蛔虫成虫来源的抗原具有作为常规抗原激活人PBMC的能力，这可能归因于它们的交叉反应性，这可能参与宿主对蠕虫感染的防御。
