Długosz Ewa, Wasyl Katarzyna, Klockiewicz Maciej, Wiśniewski Marcin
Division of Parasitology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786, Warsaw, Poland,
Parasitol Res. 2015 Sep;114(9):3365-71. doi: 10.1007/s00436-015-4561-5. Epub 2015 Jun 6.
The effect of Toxocara larval antigens on cytokine secretion by mouse splenocytes was studied in vitro. Recombinant mucins were produced in Pichia pastoris yeast, and Toxocara excretory-secretory (TES) antigens were collected from in vitro culture of L2 larvae. Tc-MUC-2, Tc-MUC-3, Tc-MUC-4, and Tc-MUC-5 were expressed as glycoproteins and were specifically recognized by Toxocara canis-infected dog serum antibodies. Mouse splenocytes stimulated with recombinant mucins produced IL-5, IL-6, and TGF-β. Cell stimulation with whole TES products was more effective and resulted in secretion of IL-4, IL-5, IL-6, IL-10, and TGF-β and downregulation of TNF-α production. IFN-γ and IL-17 secretion was noted only after ConA treatment. Cells originating from infected animals produced significantly smaller amounts of these two cytokines compared to control cells, which suggests that Th1 and Th17 response in infected mice is strongly inhibited. However, splenocyte stimulation with both TES and ConA upregulated the production of IFN-γ and IL-17. This shows that TES antigens have strong immunomodulatory properties and are able to induce a broad range of effects on murine immune cells.
在体外研究了弓首蛔虫幼虫抗原对小鼠脾细胞分泌细胞因子的影响。重组粘蛋白在毕赤酵母中产生，弓首蛔虫排泄分泌（TES）抗原从L2幼虫的体外培养物中收集。Tc-MUC-2、Tc-MUC-3、Tc-MUC-4和Tc-MUC-5表达为糖蛋白，并被犬弓首蛔虫感染犬血清抗体特异性识别。用重组粘蛋白刺激的小鼠脾细胞产生IL-5、IL-6和TGF-β。用整个TES产物刺激细胞更有效，并导致IL-4、IL-5、IL-6、IL-10和TGF-β的分泌以及TNF-α产生的下调。仅在刀豆蛋白A处理后才观察到IFN-γ和IL-17的分泌。与对照细胞相比，来自感染动物的细胞产生的这两种细胞因子的量明显较少，这表明感染小鼠中的Th1和Th17反应受到强烈抑制。然而，用TES和刀豆蛋白A刺激脾细胞上调了IFN-γ和IL-17的产生。这表明TES抗原具有强大的免疫调节特性，并且能够对小鼠免疫细胞产生广泛的影响。
