Kvasnicka H M, Thiele J
Institut für Pathologie der Universität zu Köln.
Pathologe. 1995 Mar;16(2):128-38. doi: 10.1007/s002920050085.
Computer-based three-dimensional reconstruction of serially cut light microscopic sections is being increasingly used in medical and biological research. Compared with conventional two-dimensional evaluation of histological sections, complex histotopographical relationships and structural details are easy to assess and could be imposingly visualized on the graphics screen. Because of the rapid progress in modern computer hardware, uncomplicated and fast reconstruction systems are available on standard personal computers. Therefore, 3D reconstruction is applicable for a wide range of investigations that warrant 3D exploration. Own results are presented and the principles of 3D reconstruction, as well as the problems and difficulties in this new technique, are discussed. The complex ameboid morphology of megakaryocytes in bone marrow of primary (idiopathic) osteomyelofibrosis is examined with this new method in combination with selective immunohistochemical staining procedures. 3D reconstruction can visualize the complexity of cytoplasmic and nuclear formation impressively. Moreover, the models generated permit exact quantitative measurements of cell morphology. The technique presented is a powerful tool for anatomical-morphological investigations and can furthermore increase our comprehension of complex histomorphological objects.
基于计算机的连续切片光学显微镜图像三维重建在医学和生物学研究中的应用日益广泛。与传统的组织学切片二维评估相比，复杂的组织拓扑关系和结构细节易于评估，并且可以在图形屏幕上直观地呈现出来。由于现代计算机硬件的快速发展，在标准个人计算机上即可使用简单快速的重建系统。因此，三维重建适用于广泛的需要三维探索的研究。本文展示了自身的研究结果，并讨论了三维重建的原理以及这项新技术存在的问题和困难。采用这种新方法并结合选择性免疫组织化学染色程序，对原发性（特发性）骨髓纤维化患者骨髓中巨核细胞复杂的阿米巴样形态进行了研究。三维重建能够清晰地呈现细胞质和细胞核形成的复杂性。此外，所生成的模型可对细胞形态进行精确的定量测量。本文介绍的技术是解剖形态学研究的有力工具，还能增进我们对复杂组织形态学对象的理解。
