Marsal J, Tigyi G, Miledi R
Department of Psychobiology, University of California, Irvine 92717, USA.
Proc Natl Acad Sci U S A. 1995 May 23;92(11):5224-8. doi: 10.1073/pnas.92.11.5224.
A method was developed to transplant assembled nicotinic acetylcholine receptors (AcChoRs) and Cl- channels from the electric organ of Torpedo to the membrane of Xenopus oocytes. Membrane vesicles from Torpedo electroplaques were injected into the oocytes and, within a few hours, the oocyte membrane acquired AcChoRs and Cl- channels. The mechanism of expression of these receptors and channels is very different from that which follows the injection of mRNA, since the appearance of receptors after membrane injection does not require de novo protein synthesis or N-glycosylation. This, and other controls, indicate that the foreign receptor-bearing membranes fuse with the oocyte membrane and cause the appearance of functional receptors and channels. All this makes the Xenopus oocyte an even more powerful tool for studies of the structure and function of membrane proteins.
已开发出一种方法，可将组装好的烟碱型乙酰胆碱受体（AcChoRs）和氯离子通道从电鳐的电器官移植到非洲爪蟾卵母细胞的膜上。将来自电鳐电板的膜囊泡注入卵母细胞，在数小时内，卵母细胞膜就获得了AcChoRs和氯离子通道。这些受体和通道的表达机制与注射mRNA后的情况非常不同，因为膜注射后受体的出现不需要从头合成蛋白质或进行N-糖基化。这一点以及其他对照表明，携带外源受体的膜与卵母细胞膜融合，导致功能性受体和通道的出现。所有这些使得非洲爪蟾卵母细胞成为研究膜蛋白结构和功能的更强大工具。
