Guittré C, Baginski I, Le Gall G, Prave M, Trépo C, Cova L
INSERM U271, Lyon, France.
Res Vet Sci. 1995 Mar;58(2):128-32. doi: 10.1016/0034-5288(95)90065-9.
At present there is no sensitive method for the detection of rabbit haemorrhagic disease virus (RHDV), a calicivirus causing high mortality in rabbit populations. For this purpose a reverse transcriptase polymerase chain reaction (RT-PCR) was established in the N-terminal portion of the RHDV capsid region. The RT-PCR was 10(4)-fold more sensitive than ELISA testing for the detection of the virus and was able to detect as few as 12 copies of template cDNA. By using the RT-PCR test and sequencing, 96.6 to 98.7 per cent homology was demonstrated in the N-terminal portion of the capsid protein of three isolates from geographically and temporally separate outbreaks of viral haemorrhagic disease, indicating that this portion of the RHDV capsid protein is highly conserved.
目前，对于兔出血症病毒（RHDV，一种在兔群中可导致高死亡率的杯状病毒），尚无灵敏的检测方法。为此，在RHDV衣壳区域的N端部分建立了逆转录聚合酶链反应（RT-PCR）。该RT-PCR检测病毒的灵敏度比ELISA检测高10⁴倍，能够检测低至12个拷贝的模板cDNA。通过使用RT-PCR检测和测序，在地理和时间上相互独立的三次病毒性出血病爆发中分离出的三个毒株的衣壳蛋白N端部分，显示出96.6%至98.7%的同源性，这表明RHDV衣壳蛋白的这一部分高度保守。
