Nolte I, Mischke R
Clinic of Small Animals, Hannover School of Veterinary Medicine, Germany.
Res Vet Sci. 1995 Mar;58(2):190-2. doi: 10.1016/0034-5288(95)90076-4.
The effects of the long term storage of canine blood at 4 to 6 degrees C in PVC-bags containing CPDA-1 as a stabiliser on platelet aggregation and platelet counts were investigated. Aggregation induced by collagen, adenosine diphosphate or a calcium ionophore was preserved well during the first six hours, but there was then a decrease of 24 to 46 per cent in the ability of the platelets to aggregate, after which during the next three to four weeks of storage there was no further decrease in their aggregation properties. The formation of aggregates of platelets reduced the numbers of platelets counted as single thrombocytes by more than 30 per cent during the first four days. The numbers of platelets recorded varied widely with the counting method used (counting chamber or automatically) and with the ethylenediamine tetra-acetic acid content of the dilution fluid.
研究了犬血在4至6摄氏度下于含有CPDA - 1作为稳定剂的聚氯乙烯袋中长期储存对血小板聚集和血小板计数的影响。由胶原蛋白、二磷酸腺苷或钙离子载体诱导的聚集在前六个小时内保持良好，但随后血小板聚集能力下降了24%至46%，在此之后，在接下来的三到四周储存期间，其聚集特性没有进一步下降。在头四天内，血小板聚集体的形成使计为单个血小板的血小板数量减少了30%以上。记录的血小板数量因所用的计数方法（计数室或自动计数）以及稀释液中乙二胺四乙酸的含量而有很大差异。
