Suzuki T, Ui K, Shioya N, Ihara S
Department of Plastic Surgery, Kitasato University School of Medicine, Kanagawa, Japan.
Transplantation. 1995 May 15;59(9):1236-41.
Using inbred mouse strains C3H/He and BALB/c and an anti-class I MHC monoclonal antibody, we examined the graftability of mixed cultures composed of syngeneic and allogeneic mouse keratinocytes. Keratinocytes from neonatal C3H/He (syngeneic donor) and BALB/c (allogeneic donor) mice were cultured for 7 days, both individually and at the inoculum ratio of 1:1, 1:3, or 1:15. The resulting sheets of keratinocytes were implanted subcutaneously into adult C3H/He mice and morphological evaluation was made on the 4th, 7th, and 14th days after grafting. The control cultures of 100% allogeneic keratinocytes were rejected, whereas epidermal reconstruction without typical signs of rejection took place in all grafts derived from the mixed cultures, including the 1:15 cultures. However, immunofluorescence staining showed that, within the grafts, the allogeneic keratinocytes were gradually replaced by syngeneic cells. Thus, these results suggested that graftable skin substitutes may be produced by co-culturing a small amount of autologous keratinocytes with allogeneic keratinocytes, which are readily available.
我们使用近交系小鼠品系C3H/He和BALB/c以及一种抗I类MHC单克隆抗体，检测了由同基因和异基因小鼠角质形成细胞组成的混合培养物的可移植性。将新生C3H/He（同基因供体）和BALB/c（异基因供体）小鼠的角质形成细胞分别培养7天，也以1:1、1:3或1:15的接种比例混合培养。将所得的角质形成细胞片皮下植入成年C3H/He小鼠体内，并在移植后第4天、第7天和第14天进行形态学评估。100%异基因角质形成细胞的对照培养物被排斥，而所有来自混合培养物（包括1:15培养物）的移植物均发生了无典型排斥迹象的表皮重建。然而，免疫荧光染色显示，在移植物内，异基因角质形成细胞逐渐被同基因细胞取代。因此，这些结果表明，通过将少量自体角质形成细胞与易于获得的异基因角质形成细胞共培养，可能产生可移植的皮肤替代物。
