Rouabhia M, Germain L, Bergeron J, Auger F A
Laboratoire de Recherche des Grands Brûlés/LOEX, Hôpital du St. Sacrement, Québec, Canada.
Transplantation. 1995 May 15;59(9):1229-35.
Organ transplantation has progressed rapidly during the last decades. Furthermore, tissue engineering has and will continue to enlarge the scope of organ grafting. Thus, severe skin wounds, as observed in large burn trauma patients, can now be permanently treated with cultured autologous epithelial sheets. However, the time required for autologous cell growth is a major limitation. We propose to alleviate this pitfall through a novel chimeric (allogeneic-syngeneic) epithelial cell culture technique. These chimeric epidermal grafts implanted in an animal model have been shown to allow the reappearance of a histologically normal epidermal coverage similar to simultaneously performed isografts. The regenerated epidermis contained only syngeneic keratinocytes. Thus, allogeneic cells were eliminated passively. This new culture technology could find multiple applications, notably in various skin disease therapies.
在过去几十年中，器官移植取得了迅速进展。此外，组织工程已经并将继续扩大器官移植的范围。因此，在大面积烧伤创伤患者中观察到的严重皮肤伤口，现在可以用培养的自体上皮片进行永久性治疗。然而，自体细胞生长所需的时间是一个主要限制因素。我们建议通过一种新型的嵌合（同种异体-同基因）上皮细胞培养技术来缓解这一缺陷。已证明，植入动物模型中的这些嵌合表皮移植物能够使组织学上正常的表皮覆盖重新出现，类似于同时进行的同基因移植。再生表皮仅含有同基因角质形成细胞。因此，异体细胞被被动清除。这种新的培养技术可能有多种应用，特别是在各种皮肤病治疗中。
