Kim Y, Morris M D
University of Michigan, Department of Chemistry, Ann Arbor 48109-1055, USA.
Anal Chem. 1995 Mar 1;67(5):784-6. doi: 10.1021/ac00101a002.
Pulsed field capillary electrophoresis in ultradilute solutions of sieving polymers has been used to separate nucleic acid fragments as long as 1.6 million base pairs. Hydroxyethyl cellulose solutions are used for separations in the size range 8000-50,000 base pairs. Longer chain nucleic acids are separated in mixed hydroxyethyl cellulose/poly(ethylene oxide) solutions. Separations are rapid (12-13 min).
在筛分聚合物的超稀溶液中，脉冲场毛细管电泳已被用于分离长达160万个碱基对的核酸片段。羟乙基纤维素溶液用于分离8000 - 50000个碱基对大小范围内的片段。更长链的核酸在羟乙基纤维素/聚环氧乙烷混合溶液中进行分离。分离速度很快（12 - 13分钟）。
