Metzger H, Heuber S, Steinacker A, Strüber J
Adv Exp Med Biol. 1977;94:49-55. doi: 10.1007/978-1-4684-8890-6_8.
A combined physiological and histological technique was developed in order to determine the exact position of the tip of a microelectrode used for PO2 measurements within the rat brain cortex. Using this method it was possible both to identify the tissue layer where PO2 measurements had been performed as well as obtain information about the blood capillaries within the surrounding tissue. After the PO2 had been measured small quantities of dye (Alcian Blue 8 GX) were injected through a micropipette attached to the PO2 microelectrode (tip-tip distance 2-5 micron). Subsequently the tissue was fixed by perfusion, 5 micron sections were cut and stained with Cresylviolet. The points where measurements had been made could be seen under a light microscope as green coloured spots of about 100 micron in diameter. The capillary pattern could be demonstrated by silver nitrate perfusion. Information about the capillaries, such as length, distance and the gas exchange surface were obtained via computer analysis of a television image. This method was used to analyse the O2 supply of the optical cortex of the rat. Correlation of the local PO2 distribution with the capillary data revealed differences between the archi- and the neocortex of the rat brain.
为了确定用于大鼠脑皮质内PO2测量的微电极尖端的确切位置，开发了一种生理与组织学相结合的技术。使用这种方法，既可以确定进行PO2测量的组织层，也能够获取周围组织内毛细血管的相关信息。在测量PO2之后，通过连接到PO2微电极（尖端-尖端距离为2-5微米）的微量移液器注入少量染料（阿尔辛蓝8GX）。随后通过灌注固定组织，切成5微米厚的切片并用甲酚紫染色。在光学显微镜下，可以将测量点看作直径约100微米的绿色斑点。毛细血管模式可通过硝酸银灌注显示出来。通过对电视图像进行计算机分析，获取有关毛细血管的信息，如长度、距离和气体交换表面等。该方法用于分析大鼠视觉皮质的氧气供应情况。局部PO2分布与毛细血管数据的相关性揭示了大鼠脑古皮质和新皮质之间的差异。
