Tsukada T, Eguchi K, Migita K, Kawabe Y, Kawakami A, Matsuoka N, Takashima H, Mizokami A, Nagataki S
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Biochem Biophys Res Commun. 1995 May 25;210(3):1076-82. doi: 10.1006/bbrc.1995.1766.
Transforming growth factor beta 1 (TGF-beta 1) is a potent growth inhibitor of human umbilical vein endothelial cells (HUVEC). We investigated whether apoptosis is involved in the TGF-beta 1 mediated HUVEC growth inhibition. Using immunofluorescence microscopy and staining by propidium iodide, we showed HUVEC treated with TGF-beta 1 contained characteristic apoptotic nuclei with condensation of chromatin. FACS analysis demonstrated that the number of cells with hypodiploid DNA contents were increased in HUVEC treated with TGF-beta 1. The DNA fragmentation assay exhibited typical ladder formation in TGF-beta 1-treated HUVEC. Using Western blot analysis, the expression of bcl-2 on HUVEC was down-regulated by TGF-beta 1-treatment. These findings suggest that TGF-beta 1 regulates the growth of HUVEC by inducing apoptosis accompanied with down-regulation of bcl-2 expression.
转化生长因子β1（TGF-β1）是一种对人脐静脉内皮细胞（HUVEC）具有强大抑制作用的生长因子。我们研究了细胞凋亡是否参与TGF-β1介导的HUVEC生长抑制过程。通过免疫荧光显微镜检查和碘化丙啶染色，我们发现用TGF-β1处理的HUVEC含有具有染色质凝聚特征的凋亡细胞核。流式细胞仪分析表明，用TGF-β1处理的HUVEC中DNA含量为亚二倍体的细胞数量增加。DNA片段化分析显示，在经TGF-β1处理的HUVEC中出现了典型的梯形条带。使用蛋白质印迹分析，TGF-β1处理可下调HUVEC上bcl-2的表达。这些发现表明，TGF-β1通过诱导细胞凋亡并伴随bcl-2表达下调来调节HUVEC的生长。
