Drahota Z, Saf P, Rauchová H, Kalous M
Institute of Physiology, Academy of Sciences of the Czech Republic, Praha.
Biochem Biophys Res Commun. 1995 May 25;210(3):760-5. doi: 10.1006/bbrc.1995.1724.
The inhibitory effect of mercapto- and chlorodicarbanonaborates on mitochondrial, FAD-linked, L-glycerol-3-phosphate dehydrogenase activity was tested. The mercaptodicarbanonaborate was found to be a more potent inhibitor than the chloro derivative. The Ki of mercapto- and chlorodicarbanonaborates were 0.8 M and 480 M, respectively. Whereas the mercaptodicarbanonaborate inhibited the enzyme activity competitively, the inhibitory effect of chloro derivative was noncompetitive. The inhibitory effect of mercaptodicarbanonaborate was completely prevented by addition of bovine serum albumin.
测试了巯基二碳硼烷和氯代二碳硼烷对线粒体中与黄素腺嘌呤二核苷酸（FAD）相关的L-甘油-3-磷酸脱氢酶活性的抑制作用。发现巯基二碳硼烷是比氯代衍生物更强效的抑制剂。巯基二碳硼烷和氯代二碳硼烷的抑制常数（Ki）分别为0.8 M和480 M。巯基二碳硼烷竞争性抑制酶活性，而氯代衍生物的抑制作用是非竞争性的。添加牛血清白蛋白可完全消除巯基二碳硼烷的抑制作用。
