Rauchová H, Beleznai Z, Drahota Z
Institute of Physiology, Czechoslovak Academy of Sciences, Praha.
Biochem Mol Biol Int. 1993 May;30(1):139-48.
In brown adipose tissue mitochondria, the influence of free fatty acids on FAD-linked L-glycerol-3-phosphate dehydrogenase was investigated using either hydrophilic or hydrophobic electron acceptors. The apparent kinetic parameters were determined for substrate and electron acceptors in the presence of different concentrations of oleic acid. In contrast to the L-glycerol-3-phosphate dehydrogenase enzyme from mitochondrial hyperthyroid rat liver, the brown adipose tissue enzyme shows only a single detectable L-glycerol-3-phosphate binding site. The inhibition is of competitive type for L-glycerol-3-phosphate using either hydrophilic or hydrophobic electron acceptors, while for electron acceptors non-competitive or uncompetitive inhibition was determined, respectively.
在棕色脂肪组织线粒体中，使用亲水性或疏水性电子受体研究了游离脂肪酸对与黄素腺嘌呤二核苷酸（FAD）相连的L-3-磷酸甘油脱氢酶的影响。在不同浓度油酸存在的情况下，测定了底物和电子受体的表观动力学参数。与来自线粒体甲状腺功能亢进大鼠肝脏的L-3-磷酸甘油脱氢酶不同，棕色脂肪组织中的该酶仅显示出一个可检测到的L-3-磷酸甘油结合位点。使用亲水性或疏水性电子受体时，对L-3-磷酸甘油的抑制作用均为竞争性类型，而对于电子受体，分别测定为非竞争性或反竞争性抑制。
