从人类粪便标本中分离单核细胞增生李斯特菌：使用选择性培养基PALCAM和L-PALCAMY的实验
Listeria monocytogenes isolation from human faecal specimens: experiments with the selective media, PALCAM and L-PALCAMY.
作者信息
Jensen A
机构信息
Department of Clinical Microbiology, Statens Seruminstitut, Copenhagen, Denmark.
出版信息
Lett Appl Microbiol. 1993 Jan;16(1):32-5. doi: 10.1111/j.1472-765x.1993.tb01366.x.
The selective media PALCAM and L-PALCAMY were evaluated for their potential ability to detect Listeria monocytogenes in faeces. Recovery on PALCAM was almost total, and similar at 30 degrees C and 35 degrees C with or without CO2 incubation. Warm enrichment in L-PALCAMY was necessary in order to detect low numbers (< 10(2)/ml faeces). Faeces in excess of 0.25 ml/10 ml L-PALCAMY was inhibitory. The results point to L-PALCAMY and PALCAM as an epidemiological tool.
评估了选择性培养基PALCAM和L-PALCAMY检测粪便中单核细胞增生李斯特菌的潜在能力。在PALCAM上的回收率几乎是100%，在30℃和35℃下，无论有无二氧化碳培养，回收率相似。为了检测低数量（<10²/ml粪便）的单核细胞增生李斯特菌，在L-PALCAMY中进行温育富集是必要的。超过0.25 ml/10 ml L-PALCAMY的粪便具有抑制作用。结果表明L-PALCAMY和PALCAM可作为一种流行病学工具。
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