从粪便和生物样本中分离单核细胞增生李斯特菌的选择性富集程序。
Selective-enrichment procedure for isolation of Listeria monocytogenes from fecal and biologic specimens.
作者信息
Doyle M P, Schoeni J L
出版信息
Appl Environ Microbiol. 1986 May;51(5):1127-9. doi: 10.1128/aem.51.5.1127-1129.1986.
Abstract
A selective-enrichment procedure (SEP) was developed to isolate Listeria monocytogenes from fecal and biologic specimens. This procedure was compared with direct plating with McBride listeria agar and 2-, 4-, and 8-week cold-enrichment procedures in recovering L. monocytogenes from mouse fecal, liver, and brain specimens. Although the SEP occasionally did not isolate the organism from specimens proved positive by the other procedures, the SEP isolated L. monocytogenes from about two and five times as many specimens as the cold-enrichment and direct-plating procedures, respectively.
摘要
已开发出一种选择性富集程序（SEP），用于从粪便和生物标本中分离单核细胞增生李斯特菌。将该程序与使用麦克布赖德李斯特菌琼脂直接平板接种法以及2周、4周和8周冷富集程序进行比较，以从小鼠粪便、肝脏和脑标本中回收单核细胞增生李斯特菌。尽管SEP偶尔无法从经其他程序证明为阳性的标本中分离出该菌，但SEP分别从比冷富集程序和直接平板接种法多约两倍和五倍的标本中分离出了单核细胞增生李斯特菌。
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