Mukataka S, Negishi S, Sato S, Takahashi J
Institute of Applied Biochemistry, University of Tsukuba, Japan.
Enzyme Microb Technol. 1993 Mar;15(3):229-33. doi: 10.1016/0141-0229(93)90142-o.
The activity of immobilized glucoamylase was remarkably increased by presoaking treatment of the supports in soluble starch solution. Pig bone (PB) particles-100 showed the largest substrate presoaking effect among some representative support materials, increasing the activity of immobilized glucoamylase by 10 times. The improvement in the activity was due to the increase in the specific activity of immobilized proteins. In order to get sufficient substrate presoaking effect, a rapid crosslinking treatment of the enzyme and the substrate-presoaked support was required. The glucoamylase immobilized on PB sheet was very stable and gave a high starch hydrolysis of DE95 (dextrose equivalent) for about 1 month in continuous process.
通过将载体在可溶性淀粉溶液中预浸泡处理，固定化葡萄糖淀粉酶的活性显著提高。在一些代表性载体材料中，猪骨（PB）颗粒-100表现出最大的底物预浸泡效果，使固定化葡萄糖淀粉酶的活性提高了10倍。活性的提高归因于固定化蛋白质比活性的增加。为了获得足够的底物预浸泡效果，需要对酶和预浸泡底物的载体进行快速交联处理。固定在PB片上的葡萄糖淀粉酶非常稳定，在连续过程中，能在约1个月的时间内使淀粉水解达到DE95（葡萄糖当量）的高水解率。
