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Protein engineering of subtilisins to improve stability in detergent formulations.

作者信息

von der Osten C, Branner S, Hastrup S, Hedegaard L, Rasmussen M D, Bisgård-Frantzen H, Carlsen S, Mikkelsen J M

机构信息

Novo-Nordisk A/S, Bagsvaerd, Denmark.

出版信息

J Biotechnol. 1993 Mar;28(1):55-68. doi: 10.1016/0168-1656(93)90125-7.

DOI:10.1016/0168-1656(93)90125-7
PMID:7763525
Abstract

Microbial proteases are used extensively in a large number of industrial processes and most importantly in detergent formulations facilitating the removal of proteinaceous stains. Site-directed mutagenesis has been employed in the construction of subtilisin variants with improved storage and oxidation stabilities. It is shown that in spite of significant structural homology between subtilisins subjected to protein engineering the effects of specific mutations can be quite different. Mutations that stabilize one subtilisin may destabilize another.

摘要

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