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本文引用的文献

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Crystal structures of the alkaline proteases savinase and esperase from Bacillus lentus.迟缓芽孢杆菌碱性蛋白酶savinase和esperase的晶体结构。
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A new method for integration and stable DNA amplification in poorly transformable bacilli.一种用于整合及在难转化杆菌中实现稳定DNA扩增的新方法。
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Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies.pE194的核苷酸序列和功能图谱，pE194是一种质粒，可产生对大环内酯类、林可酰胺类和B型链阳菌素抗体的诱导抗性。
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Plasmid replication stimulates DNA recombination in Bacillus subtilis.质粒复制刺激枯草芽孢杆菌中的DNA重组。
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Stability of reiterated sequences in the Bacillus subtilis chromosome.枯草芽孢杆菌染色体中重复序列的稳定性
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Cloning of aldB, which encodes alpha-acetolactate decarboxylase, an exoenzyme from Bacillus brevis.编码α-乙酰乳酸脱羧酶（一种来自短短芽孢杆菌的胞外酶）的aldB基因的克隆。
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迟缓芽孢杆菌碱性蛋白酶基因的克隆、测序及利用改进技术在迟缓芽孢杆菌染色体上对该基因的扩增。

Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique.

作者信息

Jørgensen P L, Tangney M, Pedersen P E, Hastrup S, Diderichsen B, Jørgensen S T

机构信息

Bacterial Gene Technology, Novo Nordisk A/S, 2880 Bagsvaerd, Copenhagen, Denmark.

出版信息

Appl Environ Microbiol. 2000 Feb;66(2):825-7. doi: 10.1128/AEM.66.2.825-827.2000.

DOI:10.1128/AEM.66.2.825-827.2000
PMID:10653758
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC91903/
Abstract

A gene encoding an alkaline protease was cloned from an alkalophilic bacillus, and its nucleotide sequence was determined. The cloned gene was used to increase the copy number of the protease gene on the chromosome by an improved gene amplification technique.

摘要

从嗜碱芽孢杆菌中克隆出一个编码碱性蛋白酶的基因，并测定了其核苷酸序列。利用一种改进的基因扩增技术，使克隆基因用于增加染色体上蛋白酶基因的拷贝数。