迟缓芽孢杆菌碱性蛋白酶基因的克隆、测序及利用改进技术在迟缓芽孢杆菌染色体上对该基因的扩增。
Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique.
作者信息
Jørgensen P L, Tangney M, Pedersen P E, Hastrup S, Diderichsen B, Jørgensen S T
机构信息
Bacterial Gene Technology, Novo Nordisk A/S, 2880 Bagsvaerd, Copenhagen, Denmark.
出版信息
Appl Environ Microbiol. 2000 Feb;66(2):825-7. doi: 10.1128/AEM.66.2.825-827.2000.
Abstract
A gene encoding an alkaline protease was cloned from an alkalophilic bacillus, and its nucleotide sequence was determined. The cloned gene was used to increase the copy number of the protease gene on the chromosome by an improved gene amplification technique.
摘要
从嗜碱芽孢杆菌中克隆出一个编码碱性蛋白酶的基因，并测定了其核苷酸序列。利用一种改进的基因扩增技术，使克隆基因用于增加染色体上蛋白酶基因的拷贝数。
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