Preparation and properties of RNase T1 immobilized on aminoethyl Bio-Gel P-2.
作者信息
Gite S, Shankar V
机构信息
Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
出版信息
J Biotechnol. 1993 Apr;28(2-3):339-45. doi: 10.1016/0168-1656(93)90181-l.
PMID:7763565
Abstract
The partially purified RNase T1, when coupled to glutaraldehyde activated aminoethyl Bio-Gel P-2, retained 22-24% activity of the soluble enzyme. Immobilization resulted in an increase in the optimum temperature and temperature stability, but it did not affect the pH optimum. Km and Vmax decreased as a result of immobilization. The bound enzyme showed high stability to repeated use and storage.
摘要
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