Shirley B A, Laurents D V
Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843.
J Biochem Biophys Methods. 1990 Mar;20(3):181-8. doi: 10.1016/0165-022x(90)90076-o.
A protocol for the rapid purification of ribonuclease T1 expressed from a chemically synthesized gene cloned into Escherichia coli is described. QAE ion-exchange and Sephadex G-50 chromatography are used to give over 300 mg (88% yield) of pure ribonuclease T1 from 61 of liquid culture in 3 days. We also report a new absorption coefficient for RNase T1: E1%278 nm = 15.4.
