Shinano H, Tsuneda S, Saito K, Furusaki S, Sugo T
Department of Chemical Engineering, Faculty of Engineering, University of Tokyo, Japan.
Biotechnol Prog. 1993 Mar-Apr;9(2):193-8. doi: 10.1021/bp00020a012.
A microporous hollow fiber containing a sulfopropyl (SP) group as a strongly acidic cation-exchange group was prepared by radiation-induced graft polymerization of glycidyl methacrylate, followed by hydrolysis of the resulting epoxide group into a diol, and then conversion of the diol into the SP group. The SP group density of the resulting hollow fiber ranged from 0.21 to 0.84 mol/kg of dry fiber with a pure water flux of 2.7 m/h at a filtration pressure of 0.1 MPa. Lysozyme adsorption was examined during permeation of the lysozyme solution (pH 6) through the pores across a microporous cation-exchange hollow fiber. The lysozyme concentration of the effluent penetrating the outside of the hollow fiber did not change irrespective of the residence time of the solution across the hollow fiber, which was indicative of the negligible diffusional resistance of lysozyme to the SP group. The binding capacity of lysozyme to the fiber was constant in this range of SP group density. For comparison, the adsorption characteristics of a cupric chloride solution during permeation were also determined. The binding capacity of Cu to the fiber increased linearly with increasing SP group density, because cupric ions of a smaller size than lysozyme can invade the depths of the grafted polymer branches formed in the amorphous domain of the polymer matrix.
通过甲基丙烯酸缩水甘油酯的辐射诱导接枝聚合反应制备了一种含有作为强酸性阳离子交换基团的磺丙基（SP）基团的微孔中空纤维，然后将所得的环氧基团水解成二醇，再将二醇转化为SP基团。所得中空纤维的SP基团密度为0.21至0.84摩尔/千克干纤维，在0.1兆帕的过滤压力下纯水通量为2.7米/小时。在溶菌酶溶液（pH 6）通过微孔阳离子交换中空纤维的孔进行渗透的过程中，对溶菌酶的吸附进行了检测。穿透中空纤维外部的流出物中的溶菌酶浓度不随溶液穿过中空纤维的停留时间而变化，这表明溶菌酶对SP基团的扩散阻力可忽略不计。在该SP基团密度范围内，溶菌酶与纤维的结合能力是恒定的。为作比较，还测定了氯化铜溶液在渗透过程中的吸附特性。Cu与纤维的结合能力随SP基团密度的增加而线性增加，因为尺寸比溶菌酶小的铜离子可以侵入在聚合物基质的无定形区域中形成的接枝聚合物分支的深处。
