Iwata H, Saito K, Furusaki S, Sugo T, Okamoto J
Department of Chemical Engineering, Faculty of Engineering, University of Tokyo, Japan.
Biotechnol Prog. 1991 Sep-Oct;7(5):412-8. doi: 10.1021/bp00011a005.
An immobilized metal affinity (IMA) hollow-fiber membrane was prepared by radiation-induced graft polymerization of glycidyl methacrylate (GMA) onto a porous polyethylene hollow fiber, followed by chemical conversion of the produced epoxide group into an iminodiacetate (IDA) group and its chelation with copper(II) ion. The IDA hollow fiber, whose degree of GMA grafting was 120%, was found to retain 0.42 mol of Cu ion/kg of dry weight of the resulting IMA hollow fiber. The pure water flux of the affinity membrane was 0.90 m/h at a filtration pressure of 1 x 10(5) Pa. The 0.1 g/L L-histidyl-L-leucine (His-Leu) solution permeated across the IMA hollow fiber, whose inner diameter and thickness were 0.78 and 0.365 mm, respectively, at a prescribed filtration pressure ranging from 0.2 x 10(5) to 1.0 x 10(5) Pa. The adsorption of His-Leu during permeation of the solution showed that the overall adsorption rate was independent of the filtration pressure, i.e., the residence time, because of the negligible diffusional resistance of His-Leu to the pseudobioaffinity ligand located on the pore surface of the membrane. No deterioration in the adsorption capacity was observed after five cycles of His-Leu adsorption, its elution, and reimmobilization of copper. The adsorption isotherm of bovine serum albumin (BSA) on the IMA hollow fiber was measured and compared with that for the conventional agarose-based bead containing the IDA-Cu ligand.(ABSTRACT TRUNCATED AT 250 WORDS)
通过将甲基丙烯酸缩水甘油酯（GMA）辐射诱导接枝聚合到多孔聚乙烯中空纤维上，制备了一种固定化金属亲和（IMA）中空纤维膜，随后将生成的环氧基团化学转化为亚氨基二乙酸（IDA）基团，并使其与铜（II）离子螯合。发现GMA接枝度为120%的IDA中空纤维每千克所得IMA中空纤维干重保留0.42摩尔铜离子。在1×10⁵帕的过滤压力下，亲和膜的纯水通量为0.90米/小时。在规定的0.2×10⁵至1.0×10⁵帕过滤压力范围内，0.1克/升的L-组氨酰-L-亮氨酸（His-Leu）溶液透过内径和厚度分别为0.78和0.365毫米的IMA中空纤维。溶液渗透过程中His-Leu的吸附表明，由于His-Leu对位于膜孔表面的假生物亲和配体的扩散阻力可忽略不计，总吸附速率与过滤压力即停留时间无关。在His-Leu吸附、洗脱和铜重新固定五个循环后，未观察到吸附容量下降。测量了牛血清白蛋白（BSA）在IMA中空纤维上的吸附等温线，并与含有IDA-Cu配体的传统琼脂糖基珠的吸附等温线进行了比较。（摘要截短于250字）
