Cattaneo M V, Luong J H
Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec.
Enzyme Microb Technol. 1993 May;15(5):424-8. doi: 10.1016/0141-0229(93)90130-t.
A chemiluminescence fiber optic system coupled to flow injection analysis (FIA) and ion exchange chromatography has been developed for determining glucose in blood and urine. Immobilized glucose oxidase acted on beta-D-glucose to produce hydrogen peroxide, which was then reacted with luminol in the presence of ferricyanide to produce a light signal. Endogenous ascorbic acid and uric acid present in urine or blood samples were effectively retained by an upstream acetate anion exchanger. In addition, acetaminophen could also be adsorbed by this ion exchanger. The detection system exhibited a sensitivity of 1.315 +/- 0.044 RU microM-1 for glucose with a minimum detection level of 1 microM. When applied for the determination of urinary and blood glucose levels, the results obtained compared well with those of the reference hexokinase assay. Immobilized glucose oxidase was reused for over 500 analyses without losing its original activity. A conservative estimate for the reuse of the acetate ion exchange column was about 100 analyses.
已开发出一种与流动注射分析（FIA）和离子交换色谱联用的化学发光光纤系统，用于测定血液和尿液中的葡萄糖。固定化葡萄糖氧化酶作用于β-D-葡萄糖产生过氧化氢，然后过氧化氢在铁氰化物存在下与鲁米诺反应产生光信号。尿液或血液样本中存在的内源性抗坏血酸和尿酸被上游的醋酸根阴离子交换剂有效保留。此外，对乙酰氨基酚也可被该离子交换剂吸附。该检测系统对葡萄糖的灵敏度为1.315±0.044 RU μM-1，最低检测水平为1 μM。用于测定尿液和血液葡萄糖水平时，所得结果与参考己糖激酶测定法的结果相比良好。固定化葡萄糖氧化酶可重复使用500多次而不失其原有活性。醋酸根离子交换柱重复使用的保守估计约为100次分析。
