Watabe S, Abe K, Hirata A, Emori Y, Watanabe M, Arai S
Department of Agricultural Chemistry, University of Tokyo, Japan.
Biosci Biotechnol Biochem. 1993 Apr;57(4):603-6. doi: 10.1271/bbb.57.603.
The ice nucleation-active protein of Erwinia ananas IN-10 (inaA protein) was over-expressed as inclusion bodies in Escherichia coli in a yield of 15.3 mg of inaA protein from 60 mg of bacterial cells on a dry-matter basis. The inaA protein was purified from the inclusion bodies by solubilization with detergents to obtain a protein preparation free from sugar and lipid. This preparation had a distinct ice nucleation activity, indicating that the inaA protein per se is able to act as a nucleus.
